Pages

Showing posts with label Transvaginal oocyte retrieval. Show all posts
Showing posts with label Transvaginal oocyte retrieval. Show all posts

Atlas Of IVF Embryos - Dr Sai, Senior Embryologist, Malpani Infertility Clinic

Posted by nurul Wednesday, March 9, 2011 0 comments
Unfortunately, most patients doing IVF treatment are quite clueless about the most important end-result of the IVF cycle - the embryos ! This atlas will help you understand what your eggs and embryos should look like, so you have a better understanding of what happens in the IVF lab !

DAY 0 ( the day of egg collection)

MATURE OOCYTE CUMULUS COMPLEXES



THESE ARE MATURE OOCYTE CUMULUS COMPLEXES, IDENTIFIED IN THE FOLLICULAR FLUID ASPIRATED DURING EGG COLLECTION.

POST MATURE OCCYTE CUMULUS COMPLEX

STRIPPED EGGS
MATURE EGGS :


THIS IS A MATURE EGG (SURROUNDING CUMULUS CELLS HAVE BEEN STRIPPED OFF)

POLAR BODY AT 12 O CLOCK POSITION INDICATES THAT THIS IS A MATURE EGG AT METAPHASE II
STRIPPING IS MANDATORY FOR PERFORMING ICSI ON THEM.
CUMULUS NEED NOT BE STRIPPED FOR CONVENTIONAL IVF.



THIS EGG IS NOT A MATURE EGG. BUT A TINY POLAR BODY WHICH IS PROTRUDING OUT AT 12 O CLOCK POSITION, INDICATES THAT IT WILL SOON BECOME MATURE.

IMMATURE EGGS


Metaphase I egg

We can perform ICSI on Metaphase I eggs. The fertilization rate for Metaphase I eggs is 50 %.


Egg with Germinal Vescicle

THIS EGG HAS A CLEAR GERMINAL VESCICLE IN IT, WHICH INDICATES IT IS AN IMMATURE EGG. WE CANNOT DO ICSI ON THIS EGG. WE CAN USE IVM ( IN VITRO MATURATION) FOR THESE EGGS. AFTER 24 HOURS, SOME OF THEM MAY MATURE AND BECOME M2 ( METAPHASE II) AT WHICH TIME WE CAN DO ICSI FOR THEM

ABNORMAL EGGS


EGG WITH ABNORMAL ZONA EGG WITH ABNORMAL CYTOPLASM.


EGG WITH 2 POLAR BODIES.

POOR QUALITY EGGS

EGGS WITH DARK CENTRAL GRANULATION IN CYTOPLASM.


EGG WITH VACUOLATION IN CYTOPLASM

UNFORTUNATELY, THERE IS NO WAY WE CAN PREDICT THE QUALITY OF EGGS BEFORE WE STRIP THEM.
THIS IS WHY STRIPPING THE EGGS FOR ICSI PROVIDES US WITH VALUABLE INFORMATION ABOUT EGG QUALITY !

DAY 1
DAY 1 EMBRYOS ( ALSO CALLED ZYGOTES) ARE SCORED CONSIDERING

  • THE PRESCENCE OF CYTOPLASMIC HALO
  • NUCLEAR SIZE AND ALIGNMENT, NUCLEOLI NUMBER AND DISTRIBUTION.
  • PRESCENCE OF CYTOPLASMIC HALO


PICTURE A – HALO POSITIVE ZYGOTE PICTURE B – HALO NEGATIVE ZYGOTE

2) NUCLEOLI NUMBER AND DISTRIBUTION

ZYGOTES ARE SCORED ACCORDING TO THE Z-SCORING SYSTEM.

  • PICTURE A IS Z1 ZYGOTE WITH EQUAL NUMBER OF NUCLEOLI, ALIGNED AT THE PRONUCLEAR JUNCTION.
  • PICTURE B IS A Z2 ZYGOTE WITH EQUAL NUMBER OF NUCLEOLI, SCATTERED IN THE TWO NUCLEI.
  • PICTURE C IS A Z3 ZYGOTE WITH UNEQUAL NUMBER (DIFFERENCE OF MORE THAN 2 NUCLEUS) AND / OR SIZES OF NUCLEOLI.
  • PICTURE D IS A Z4 ZYGOTE WITH SEPERATED PRONUCLEI AND UNEQUAL NUMBER OF NUCLEOLI SCATTERED IN THE TWO NUCLEI.

NORMAL ZYGOTE


Z1 EMBRYO

POOR QUALITY ZYGOTE

Z3 EMBRYO

ABNORMAL ZYGOTES

ZYGOTE WITH 3 PRONUCLEI


ZYGOTE WITH 2 PRONUCLEI WITHOUT NUCLEOLI


ZYGOTE WITH 1 PRONUCLEUS

Enhanced by Zemanta

How Can You Be Sure Your IVF Clinic Is Any Good?

Posted by nurul Saturday, December 25, 2010 0 comments

One of the major decisions infertile patients have to make is - How to pick a really good IVF clinic? The majority of IVF treatment centers appear to be identical on paper, and their websites are quite similar (because some unethical doctors will unashamedly copy and paste content from our website!). Many have happy patients - and most IVF specialists talk the identical talk - all of them quote high success rates and claim they are the finest! Exactly how is a poor patient supposed to separate the wheat from the chaff and assure himself that the IVF clinic he's going to is efficient? After all, while it is easy for patients to evaluate a doctor's bedside manner, it's difficult for them to make a call concerning his medical and technical proficiency.

Simply because the IVF cycle fails doesn't mean that the doctor was bad or the medical clinic was incompetent. After all, IVF carries a restricted rate of success, and only about 20% of embryos implant to become a baby. An unsuccessful IVF might just imply that the embryos failed to implant for unexplained biological causes - and isn't immediately a cause to switch your IVF clinic!

One extremely important tangible end point that all patients may use to evaluate the particular clinical proficiency of their own IVF clinic is to demand that the clinic give them photographic documentation of their own embryos. All superior treatment centers do it regularly, for a lot of factors. It records the high quality of medical attention delivered - after all, the proven fact that the clinic made embryos implies that they are skilled at super ovulation, egg retrieval and fertilization - and high quality clinics are usually proud to show their expertise! Creating embryos in vitro depends on elements that are primarily in the clinic's control - while the result of the embryo transfer, sadly, is not. This record additionally signifies that they have confidence about their clinical expertise and have nothing to conceal. This openness is extremely patient-empowering! Equally importantly, these photographs are extremely useful in circumstances when you need to get a second opinion, or to proceed to another IVF clinic.

In case your IVF clinic doesn't regularly give you with a written brief summary of your treatment cycle and photos of your embryos, then this is a warning sign! And if they refuse to share these with you even after you ask them to, this is a major black mark against them - and is a powerful indication that you need to search for another clinic. After all, why should a good clinic conceal anything?

Malpani Infertility Clinic offers IVF ( In Vitro Fertilization )Treatment to infertile couples from all over the world. Like most infertile couples, you know that IVF can maximise your chances of getting pregnant. Malpani Infertility Clinic, IVF treatment Center in India

Enhanced by Zemanta